Multiple SDS-PAGE on Vertical Electrophoresis Units.
نویسندگان
چکیده
INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-dimension SDS-PAGE systems. This protocol presents a method for preparing and running multiple vertical SDS gels.
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ورودعنوان ژورنال:
- CSH protocols
دوره 2006 1 شماره
صفحات -
تاریخ انتشار 2006